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As the First International Conference on Water and Ions in Biological Systems (Bucharest, June 25-27, 1980) was appreciated as a success, a second one was organized in the fall of the year 1982 under the sponsorship of the United Nations Educational, Scientific and Cultural Organization (UNESCO), the Romanian Academy of Medical Sciences, the Romanian Biophysical Society (Union of Societies for Medical Sciences in the Socialist Republic of Romania) and in co- operation with the International Union for Pure and Applied Bio- physics (IUPAB). The responsibility for the scientific program and organization of the Second Conference on Water fell on an International Scientific Committee which included Prof. J. Tigyi (Pees), President of the UNESCO Expert Committee on Biophysics, Prof. K. Wuthrich, Secretary General of IUPAB and Prof. H. Eisenberg, (member of the IUPAB Council) under the guidance of an Executive Board whose members were Prof. J. Jaz (representative of UNESCO), Prof. B. Pullman (Vice- President of IUPAB) and Prof. V. Vasilescu (President of the Romanian Biophysical Society). The Meeting was attended by more than 250 specialists including 150 Romanian participants and others from Bulgaria, Czechoslovakia, England, the Federal Republic of Germany, the German Democratic Republic, Greece, Hungary, India, Israel, Italy, Japan, the Netherlands, Nigeria, Poland, Sweden, Switzerland, USSR, USA, Venezuela, Yugoslavia. The proceedings of the Conference took place in the Medical Faculty of Bucharest. The theoretical and practical importance of the Meeting was pointed out by the speakers, among whom were Prof.
Intensive research carried out during the 1990's (known as the "Iron Age of Oceanography") provided a wealth of new information and this title, written by acknowledged experts and reviewed by international specialists, provides the authoritative and comprehensive review of the subject area.<br> <br> A joint venture between SCOR and IUPAC, it expertly addresses the current state of knowledge of the biogeochemistry of iron in seawater and covers:<br> <br> * Chemical speciation<br> * Analytical techniques<br> * Transformation of iron<br> * It includes evidence for iron limitation of primary production of High Nutrient Low Chlorophyll (HNLC) areas in the ocean<br> * Structured into a series of chapters it has been reviewed by international specialists- SCOR (Scientific Committee for Ocean Research) and IUPAC (International Union for Pure and Applied Chemistry)<br> * The final chapter summarises the conclusions of the book and discusses the priorities for future research<br> * Ideal for scientists studying the environmental impact of metals and their role in marine ecosystems; Marine Scientists and Oceanographers; Environmental Analytical Chemists
Two methods for the detection of important human pathogens, Cryptosporidium parvum and Helicobacter pylori, were investigated: a fiber optic biosensor, and real time PCR. The mechanism for specific detection in both methods is recognition of specific DNA sequences in the target organisms. The biosensor that was used, the Analyte 2000, was originally developed for the detection of chemicals. It utilizes a fiber optic wave guide that propagates an evanescent light wave of very specific wavelength. The light excites fluorescent molecules bound to the waveguide, but not in the bulk solution, which theoretically enhances signal while reducing background interference. Attempts to develop this system for the detection of DNA were not successful due to poor detection of the target molecules. An assay analogous to a sandwich immunoassay was designed for use on the Analyte 2000. Specific oligonucleotide probes were designed to bind to the waveguides via biotin-streptavidin interaction, and were used to capture the target DNA. Pure target DNA representing unique genes in the organisms were synthesized by PCR. Detection of captured DNA was then attempted using an oligonucleotide detection probe designed to bind to the target. Two detection systems were employed: an indirect signal amplification system based on biotin-tyramide deposition, or direct detection of fluorescent signal from Cy-5 molecules. In all experiments performed there was very little difference between the signal generated with or without the target molecules. Many experiments were conducted to attempt to identify reasons for the poor signal. Signal was only of any significance when target amplicons were internally labeled with Cy-5 by PCR. Real time PCR as a method to detect the pathogens was also investigated. Though the PCR technique itself is very rapid, DNA extraction and purification requires preparation time. Filtration of up to one liter of well water, followed by concentration and "cleaning" Helicobacter pylori cells by immunomagnetic separation, was used to detect H. pylori seeded in a water source. Following cell lysis, the extracted DNA could be used directly in conventional PCR targeting the 16S rRNA gene to detect less than 265 cells per liter of water. DNA purification was not required for this level of detection. Initial studies to amplify lysed cells by real time PCR indicated that an incorrect product was made. When purified DNA was used for real time PCR, the correct product was produced from DNA representing as few as 100 cells. This publication can be purchased and downloaded via Pay Per View on Water Intelligence Online - click on the Pay Per View icon below
The Pure and Real Recipe Guide is a 10 day vegetarian (and vegan) healthy eating plan. You eat only pure nutritional food grown fresh from God's Garden; to gain energy, vitality, weight loss and to detox your body. This Real Recipe Guide is designed to compliment Philips Award winning and Bestselling book series based on Daniels Dietary Plan This Recipe Book gives you step-by-step instructions for creating vegetarian dishes - recipes so delicious that even fussy eaters will enjoy! All the foods are found naturally in God's garden and include the colors of the rainbow to ensure full nutritional value. All Gluten, Dairy, Chemical and Wheat Free 10 easy-to-make breakfast recipes 10 morning and afternoon teas 10 Lunches 10 dinners 'This food plan is far and away the best and cleanest diet I have ever tried', (Vicki).
Rough Waters is a story about friends and family. It is about family ties and the strengths that come from it. There are a lot of mixed emotions and opinions from the group about how things should be done but, in the end they manage to work together. This exciting story takes place on the Flathead River in Montana, in the very beginning of August. The weather is warm and the water is rushing with all the Glacier run off that the 90+ degree weather has brought. The trip down the river is all about celebration; the ending of one era and the beginning of another for the two cousins Annie and Lindsey. It is also about new experiences and having a lot of crazy and spontaneous fun. Sometimes when you mix the two it becomes dangerous and potentially costly. If you are looking for something that is exciting to read all the way through with some hilarious exploits from the characters with a side of danger and fear, then this is the book for you. Rough Waters is an easy read and should be suitable for most audiences. This is not a gruesome horror story, but more a story about a group of friends having a fun time on the river and it takes a turn for the worst making it a story about adventure and triumph.
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